ISOLATION AND CHARACTERIZATION OF MILDIOMYCIN, A NEW NUCLEOSIDE ANTIBIOTIC SETSUO HARADA and TOYOKAZU KISHI
ثبت نشده
چکیده
A new antibiotic mildiomycin, strongly active against powdery mildew, was isolated from the culture filtrate of Streptoverticilliun: rimofaciens B-98891. It is a water-soluble basic antibiotic and was purified by ion-exchange and adsorption chromatography. The molecular formula of the purified compound was determined to be C19H30N8O9(H2O) from physical and chemical data. The UV and NMR spectra suggested that this antibiotic is a nucleoside. On acidic hydrolysis it gave 5-hydroxymethyl cytosine which has not previously been found in nucleoside antibiotics.
منابع مشابه
ISOLATION AND CHARACTERIZATION OF A NEW NUCLEOSIDE ANTIBIOTIC, AMIPURIMYCIN SETSUO HARADA and TOYOKAZU KISHI
A new antibiotic amipurimycin, active against Pyricularia oryzae in vitro and in vivo, was isolated from the culture filtrate of Streptomyces novoguineensis nov. sp. The antibiotic was purified by a combination of ion-exchange and adsorption chromatography based on its amphoteric water-soluble characteristics. Its molecular formula was estimated to be C2oH27~31N7O8.H2O. Characteristic maxima in...
متن کاملCapuramycin, a new nucleoside antibiotic. Taxonomy, fermentation, isolation and characterization.
A new antibiotic capuramycin was isolated from the culture filtrate of Streptomyces griseus 446-S3 by adsorption and partition column chromatography. Based on its chemical structure being an uracil nucleoside with a caprolactam substituent, this substance was named as capuramycin. This paper describes the taxonomy of a producing organism, fermentation, isolation, characterization and biological...
متن کاملB-1008, a new antibiotic of bacterial origin containing a spermidine moiety. Production, isolation, characterization and biological properties.
A new antibiotic, B-1008 has been isolated from the cultured broth of Pseudomonas fluorescens No. 101 B-13L. B-1008 is a water-soluble basic substance containing the spermidine moiety and possessing antibacterial activity against a wide range of bacterial species.
متن کاملIsolation, Characterization and Antibiotic Resistance of Shiga Toxin-Producing Escherichia coli in Hamburger and Evolution of Virulence Genes stx1, stx2, eaeA and hly by Multiplex PCR
Background & Objectives: Shiga toxin-producing Escherichia coli (STEC) O157:H7 have emerged as pathogens that can cause food-borne infections and severe and potentially fatal illnesses in humans. E.coli O157:H7 colonizes the digestive tract of cattle and is transmitted to humans by food and water. The objectives of this study were to characterize the prevalence of E.coli O157:H7 isolates in ham...
متن کاملIsolation, Molecular Characterization and Antibiotic Susceptibility Pattern of Pasteurella multocida Isolated from Cattle and Buffalo from Ahwaz, Iran
Pasteurella multocida is a gram-negative, opportunistic pathogen and the common inhabitant of the upper respiratory tract of domesticated and wild animal species. It is a causative agent of numerous economically important diseases worldwide, such as enzootic bronchopneumonia in ruminants and haemorrhagic septicemia in cattle and buffalo. The present study was undertaken to determine the prevale...
متن کامل